• 供应M-MLV -反转录酶

    供应M-MLV -反转录酶

  • 2016-07-01 14:47 172
  • 产品价格:面议
  • 发货地址:上海市闵行区包装说明:不限
  • 产品数量:不限产品规格:不限
  • 信息编号:9688488公司编号:352240
  • 罗小姐 市场
    13000000000 (联系我请说明是在阿德采购网看到的信息)
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    产品描述
    FicoScript®M-MLV Transcriptase(反转录酶) 
    
    编号 
     规格 
     售价¥ 
    ZP00601 
     1000U 
     150 
    ZP00602 
     5000U 
     500 
    ZP00603 
     10000U 
     800 
       相关试剂 
    抽提试剂盒... 
    反转录试剂盒... 
    dNTPs... 
    Taq酶... 
    DEPC... 
    枪头、离心管... 
     
    
    FicoScript®Reverse Transcriptase(反转录酶) is an engineered version of M-MLV RT with reduced RNase H activity and increased thermal stability. The enzyme is purified to near homogeneity from E. coli containing the modified pol gene of Moloney Murine Leukemia Virus . The enzyme can be used to synthesize first-strand cDNA at temperatures up to 48°C, providing increased specificity, higher yields of cDNA, and more fulllength product than other reverse transcriptases. It can generate cDNA from 100 bp to >12 kb.It is suitable for qPCR. 
    1 、 Enzyme Storage Buffer: M-MLV Reverse Transcriptase is supplied in 20mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM 
    
    EDTA, 1mM DTT, 0.01% Nonidet P-40 and 50% glycerol. 
    
    2 、 M-MLV Reverse Transcriptase 5X Reaction Buffer: When the M-MLV Reverse Transcriptase 5X Reaction Buffer supplied with this enzyme is diluted 1:5, it has a composition of 50mM Tris-HCl (pH 8.3 25°C ), 75mM KCl, 3mM MgCl 2 and 10mM DTT. 
    
    3 、 Source: Purified from an E. coli strain expressing a recombinant clone. 
    
    4 、 Storage Conditions: Store at – 20°C . Avoid exposure to frequent temperature changes. 
    
    5 、 Unit Definition: One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C . The reaction conditions are: 50mM Tris-HCl (pH 8.3), 7mM MgCl 2 , 
    
    40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [ 3 H]dTTP, 0.025mM oligo(dT) 50 , 0.25mM poly(A) 400 and 0.01% NP-40. 
    
    6 、 Usage Note: M-MLV Reverse Transcriptase is less processive than AMV Reverse Transcriptase, and therefore, more units of the M-MLV enzyme are required to generate the same amount of cDNA as in the AMV reaction. Thus, starting with 1μg of mRNA 
    
    in a first-strand cDNA synthesis, 200 units of the M-MLV enzyme are recommended as opposed to 25 units of the AMV enzyme. 
    
    反转录酶说明书下载 (大宗用户价格面议  021-5460832)

    上海闪晶分子生物科技有限公司是以中科院生化所、细胞所、二医大的科技力量为依托的一家生物高科技企业,**从事分子生物学技术服务,主要有:实时荧光定量PCR技术服务、多肽合成、荧光探针标记、DNA合成、DNA测序、DNA克隆、全基因合成、核酸纯化试剂、生化试剂、内切酶、蛋白表达、载体构建等。

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    公司长期供应分子生物学试剂盒、荧光定量PCR、siRNA载体构建、全基因合成拼接、荧光探针修等,产品质量完全符合行业要求,被用户评为信得过产品,畅销全国各省市、自治,欢迎新老客户来选购考察!

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上海闪晶分子生物科技有限公司是以中科院生化所、细胞所、二医大的科技力量为依托的一家生物高科技企业,**从事分子生物学技术服务,主要有:实时荧光定量PCR技术服务、多肽合成、荧光探针标记、DNA合成、DNA测序、DNA克隆、全基因合成、核酸纯化试剂、生化试剂、内切酶、蛋白表达、载体构建等。
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